Human CellExp™ PDGF-BB, human recombinant

Biomolecule/Target: N/A

Synonyms: PDGFB, PDGF-B, FLJ12858, PDGF2, PDGF-2

Alternates names: N/A

Taglines: Mitogenic during early developmental stages

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5155

NCBI Gene Symbol: PDGFB

Gene Source: Human

Accession #: P01127

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg) by LAL method

Activity (Specifications/test method): The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Biological activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. ED50 was 1-10 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein has a calculated MW of 25 kDa. DTT-reduced protein migrates as a 15 kDa polypeptide and the non-reduced cystine-linked homodimer migrates as a 30 kDa protein.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: N/A

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans