Human CellExp™ SARS-CoV-2 Spike Protein (RBD; 331-524), Recombinant

Biomolecule/Target: N/A

Synonyms: Novel Coronavirus RBD Protein, 2019-nCoV Spike Protein RBD, SARS-CoV-2 S1 RBD Protein

Alternates names: Novel Coronavirus RBD Protein, 2019-nCoV Spike Protein RBD, SARS-CoV-2 S1 RBD Protein

Taglines: Glycoprotein that enables viral entry into the host cell

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 43740568

NCBI Gene Symbol: S

Gene Source: Coronavirus

Accession #: HEK 293 cells

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS - PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: ~25 kDa (6×His tag at the C-terminus)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Background Information: The spike (S) glycoprotein of coronaviruses mediates entry into receptor-expressing host cells and plays the most important role in viral attachment, fusion, and entry; therefore, it serves as an important target for monoclonal antibodies, entry inhibitors, and vaccines. The S protein has two domains S1 and S2, where S1 facilitates initial binding to the receptor and the S2 domain drives membrane fusion and eventual entry of the virus. Within the S1 protein, the highly conserved receptor-binding domain (RBD) expresses a high affinity for the Angiotensin converting enzyme 2 receptor (ACE2). Experiments have shown that the RBD fragment blocked S-protein mediated infections and suggests potential as a viral attachment or entry inhibitor against SARS-CoV-2.

Amino acid sequence: aa 331-524

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans