Human CellExp™ SARS-CoV-2 Spike RBD (N501Y), Recombinant

Biomolecule/Target: N/A

Synonyms: COVID-19 Spike RBD protein (N501Y); 2019-nCoV Spike RBD protein (N501Y); SARS-CoV-2 Mutant (N501Y); SARS-CoV-2 (N501Y) mutant protein

Alternates names: COVID-19 Spike RBD protein (N501Y); 2019-nCoV Spike RBD protein (N501Y); SARS-CoV-2 Mutant (N501Y); SARS-CoV-2 (N501Y) mutant protein

Taglines: Spike RBD (N501Y) mutant increases binding affinity to ACE2

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: N/A

Gene Source: Coronavirus

Accession #: QHD43416.1

Recombinant: 1

Source: Human cells

Purity by SDS-PAGE #: ≥ 95% by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The predicted molecular weight is 26 kDa. The protein migrates as a 33-36 kDa band under reducing conditions

Concentration: N/A

Appearance: Liquid

Physical form description: N/A

Reconstitution Instructions: N/A

Background Information: SARS-CoV-2 Spike protein is a large type I transmembrane protein composed of S1 subunit and S2 subunit. During viral infection, the receptor-binding domain (RBD) of the S1 subunit is responsible for the recognition and binding of host receptor ACE2, while the S2 subunit mediates viral cell membrane fusion. The SARS-CoV-2-S1-RBD/ACE2 interaction mediates viral entry into the target cells. The N501Y mutation in the receptor-binding domain (RBD) of SARS-CoV-2 variants increases binding affinity to ACE2.

Amino acid sequence: The target protein is expressed with the sequence Arg319-Ser541(N501Y) of SARS-CoV-2 Spike RBD protein fused with a 6xHis tag at the C-terminus.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans