Human CellExp™ SARS-CoV-2 Spike RBD (K417N, E484K, N501Y)

Biomolecule/Target: N/A

Synonyms: COVID-19 Spike RBD protein (K417N, E484K, N501Y); 2019-nCoV Spike RBD protein (K417N, E484K, N501Y); SARS-CoV-2 Mutant (K417N, E484K, N501Y); SARS-CoV-2 (K417N, E484K, N501Y) mutant protein; South African (ZA) variant (501Y.V2, B.1.351)

Alternates names: COVID-19 Spike RBD protein (K417N, E484K, N501Y); 2019-nCoV Spike RBD protein (K417N, E484K, N501Y); SARS-CoV-2 Mutant (K417N, E484K, N501Y); SARS-CoV-2 (K417N, E484K, N501Y) mutant protein; South African (ZA) variant (501Y.V2, B.1.351)

Taglines: Spike RBD variant with mutations which mildly increase receptor binding

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: N/A

NCBI Gene Symbol: N/A

Gene Source: Coronavirus

Accession #: YP_009724390.1

Recombinant: 1

Source: Hek293

Purity by SDS-PAGE #: ≥ 90% by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 35 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: N/A

Reconstitution Instructions: Reconstitute in sterile water to a concentration of 1 mg/ml.

Background Information: SARS-CoV-2 Spike protein is a large type I transmembrane protein composed of S1 subunit and S2 subunit. During viral infection, the receptor-binding domain (RBD) of the S1 subunit is responsible for the recognition and binding of host receptor ACE2, while the S2 subunit mediates viral cell membrane fusion. The SARS-CoV-2-S1-RBD/ACE2 interaction mediates viral entry into the target cells. The 501Y.V2 variant or lineage B.1.351 was first detected in South Africa. The variant contains three spike receptor binding site mutations (K417N, E484K and N501Y) that have been shown to mildly increase receptor binding.

Amino acid sequence: The target protein SARS-CoV-2 S Protein RBD (K417N, E484K, N501Y) is expressed with His tag at the C-terminus.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans