Human CellExp™ SHH, human recombinant

Biomolecule/Target: SHH

Synonyms: SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

Alternates names: SHH, HHG-1, Sonic hedgehog, HLP3, HPE3, SMMCI, TPT, TPTPS

Taglines: Hedgehog signaling pathway ligand involved in regulating vertebrate organogenesis

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 6469

NCBI Gene Symbol: SHH

Gene Source: Human

Accession #: Q15465

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/μg of protein (<1EU/μg).

Activity (Specifications/test method): Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

Biological activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 1.5-10 μg/mL.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6 × His tag at the C-terminus, has a calculated MW of 20.4 kDa. The predicted N-terminus is Cys 24. DTT-reduced protein migrates as 22 kDa.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Sonic hedgehog protein (SHH) also known as HHG-1, which belongs to the hedgehog family. SHH is expressed in fetal intestine, liver, lung, and kidney and not expressed in adult tissues. Sonic hedgehog protein can be cleavage into sonic hedgehog protein N-product and sonic hedgehog protein C-product by autolysis. SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development. The C-terminal domain displays an auto-proteolysis activity and a cholesterol transferase activity. The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans