Biovision
Human CellExp™ TNFR1 / CD120a / TNFRSF1A, human recombinant
- SKU:
- 26-7382
- Availability:
- Usually Shipped in 5 Working Days
- Storage Temperature:
- -20°C
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Description
Biomolecule/Target: TNFR1 / CD120a / TNFRSF1A
Synonyms: TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
Alternates names: TNFR1, CD120a, TNFRSF1A, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR55, TNFR60, p55, p60.
Taglines: Contributes to the induction of non - cytocidal TNF effects
Taglines: USA
Country of Animal Origin: USA
NCBI Gene ID #.: 7132
NCBI Gene Symbol: TNFRSF1A
Gene Source: Human
Accession #: P19438
Recombinant: Yes
Source: HEK293 cells
Purity by SDS-PAGE #: ≥92%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: <1 EU/μg by LAL method
Activity (Specifications/test method): N/A
Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 μg/mL (100 μl/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 μg/mL.
Results: Measured by its binding ability in a functional ELISA. Immobilized human TNFα at 10 μg/mL (100 μl/well) can bind biotinylated human TNFRSF1A. The EC50 of biotinylated human TNFRSF1A is 0.02 μg/mL.
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 22 kDa. The predicted N-terminus is Ile 22. DTT-reduced Protein migrates as 30-35 kDa due to different glycosylation.
Concentration: N/A
Appearance: Lyophilized
Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information: Tumor necrosis factor receptor 1 (TNF-R1) also known as Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), TNFAR, CD antigen CD120a, belongs to the tumor necrosis factor receptor superfamily. TNF-R1 contains one death domain and four TNFR-Cys repeats. TNF-R1 is the receptor of TNFSF2 / TNF-alpha and homotrimeric TNFSF1 / lymphotoxin - alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TNF-R1 contributes to the induction of non - cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Defects in TNFRSF1A are the cause of familial Hibernian fever (FHF).
Amino acid sequence: N/A
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans