Human CellExp™ VEGF 121, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant VEGF 121

Alternates names: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Taglines: A signaling protein involved in both vasculogenesis and angiogenesis

Taglines: USA

Country of Animal Origin: China

NCBI Gene ID #.: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: P15692

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically 2 to 8 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).

Results: 2 to 8 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 37 kDa, homodimer; 50 kDa, homotrimer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized in PBS.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Background Information: VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophage migration, neurons, cancer cells, kidney epithelial cells).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans