Human CellExp™ VEGF 165, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Vascular endothelial growth factor A, MVCD1, VEGF, VEGF165, VPF, MGC70609, Vascular Endothelial Growth Factor A precursor, Vascular Permeability Factor, VEGFA

Alternates names: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VEGF, MGC70609.

Taglines: A signaling protein involved in both vasculogenesis and angiogenesis

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: P15692

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The activity was determined by the dose-dependent stimulation of the proliferation of EA.hy926 human endothelial cells with EC50 600 ng/ml.

Biological activity: EC50 is 600 ng/ml. The activity was determined by the dose-dependent stimulation of the proliferation of EA.hy926 human endothelial cells.

Results: 600 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 45 kDa, homodimer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS pH 7.4

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute at 0.2-0.3 mg/mL in distilled water is recommended.

Background Information: Vascular Endothelial Growth Factor (VEGF) plays a key role in tumor angiogenesis in many cancers. The VEGF family consists of seven secretory glycoproteins: VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, VEGF-F and Placental Growth Factor (PlGF). The binding of VEGF and its receptors leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGFA/VEGF165 is a heparin-binding protein, which exists as a disulfide-linked homodimer. It induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Diseases associated with VEGFA/VEGF165 include microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. It is also related to the integrated Breast Cancer Pathway.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans