Human CellExp™ Fas Ligand/FasL, human recombinant

Biomolecule/Target: Fas Ligand/FasL

Synonyms: FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand

Alternates names: FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand

Taglines: Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 356

NCBI Gene Symbol: FASLG

Gene Source: Human

Accession #: P48023

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.

Results: Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.1-1.5 ng/mL in the presence of 10 μg/mL of a crosslinking antibody Mouse Anti poly-Histidine Monoclonal Antibody.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at N-terminus, has a calculated MW of 17.7 kDa. The predicted N-terminus is His. Protein migrates as 25-32 kDa in reduced SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lympho proliferative syndrome (ALPS), a childhood disorder of apoptosis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans