Human CellExp™ FLT-3 Ligand, Mouse Recombinant

Biomolecule/Target: N/A

Synonyms: FLT3LG, FL, FLT3L, Flt3 ligand

Alternates names: FLT3LG, FL, FLT3L, Flt3 ligand

Taglines: A member of a small family of growth factors that stimulate the proliferation of hematopoietic cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 14256

NCBI Gene Symbol: Flt3lg

Gene Source: Mouse

Accession #: P49772

Recombinant: FALSE

Source: HEK 293 cells

Purity by SDS-PAGE #: >90% as determined by SDS-PAGE.

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method

Activity (Specifications/test method): Immobilized Mouse Flt-3 Ligand, His Tag at 1 μg/mL (100 μL/well) can bind Human Flt-3/Flk-2 Fc Chimera with a linear range of 1-5 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. It is recommended to store at -20°C.

Background Information: FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

Amino acid sequence: AA Gly 27 - Arg 188

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans