Human CellExp™ Gremlin / GREM1, Fc Tag, Human recombinant

Biomolecule/Target: Grem1

Synonyms: GREM1, CKTSF1B1, DAND2, DRM, PIG2

Alternates names: GREM1, CKTSF1B1, DAND2, DRM, PIG2

Taglines: A TGF-β antagonist

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 23892

NCBI Gene Symbol: Grem1

Gene Source: Human

Accession #: O70326

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human BMP-2, at 2 μg/Ml (100 μL/well) can bind Human Gremlin, Fc Tag with a linear range of 0.8-80 ng/mL.

Results: N/A

Binding Capacity: Immobilized Human BMP-2, at 2 μg/Ml (100 μL/well) can bind Human Gremlin, Fc Tag with a linear range of 0.8-80 ng/mL.

Unit Definition: N/A

Molecular Weight: 44.2 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans