Human CellExp™ ICOSLG /B7-H2 /CD275, human recombinant

Biomolecule/Target: ICOSLG /B7-H2 /CD275

Synonyms: ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand.

Alternates names: ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand.

Taglines: N/A

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 23308

NCBI Gene Symbol: ICOSLG

Gene Source: Human

Accession #: O75144

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human ICOSLG / B7H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 3.2 - 400 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human ICOSLG / B7H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 3.2 - 400 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.5 kDa. The predicted N-terminus is Asp 19. DTT-reduced Protein migrates as 45-50 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans