Human CellExp™ IL-10, Human Recombinant
- Usually Shipped in 5 Working Days
- Storage Temperature:
- Shipping Conditions:
- Gel Pack
- Shelf Life:
- 12 months
Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Alternates names: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
Taglines: An anti-inflammatory cytokine
Country of Animal Origin: USA
NCBI Gene ID #.: 3586
NCBI Gene Symbol: IL10
Gene Source: Human
Accession #: P22301
Source: Human 293 cell expressed
Purity by SDS-PAGE #: > 95%
Assay #2: N/A
Endotoxin Level: < 1.0 EU per 1 μg of protein
Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using BioVision’s Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.
Biological activity: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 19 kDa with a 6×His tag at the C-terminus
Physical form description: Lyophilized
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.
Background Information: The cytokine IL-10 is a key anti-inflammatory mediator with an essential role in the development and proliferation of regulatory T cells, restricting myeloid and chronic inflammatory T cell responses. It has been shown that in solution IL-10 predominantly exists as a homodimer. However, the subunits within the dimer are non-covalently associated, although each subunit contains two intra-chain disulfide bonds. IL10 binds to its hetero-tetrameric receptor comprising IL10RA and IL10RB, which activates multiple pathways that contribute to the downstream anti-inflammatory effects. It also plays important roles in other processes such as sterile wound healing, autoimmunity, cancer, and homeostasis. Given the wide spectrum of the anti-inflammatory properties attributed to IL-10, both IL-10 enhancing and blockade strategies have been tried in a number of diseases and cancer therapy.
Amino acid sequence: Ser 19 – Asn 178
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans