Human CellExp™ IL-10, Human Recombinant

Biomolecule/Target: N/A

Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Alternates names: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Taglines: An anti-inflammatory cytokine

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3586

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P22301

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using BioVision’s Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19 kDa with a 6×His tag at the C-terminus

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Background Information: The cytokine IL-10 is a key anti-inflammatory mediator with an essential role in the development and proliferation of regulatory T cells, restricting myeloid and chronic inflammatory T cell responses. It has been shown that in solution IL-10 predominantly exists as a homodimer. However, the subunits within the dimer are non-covalently associated, although each subunit contains two intra-chain disulfide bonds. IL10 binds to its hetero-tetrameric receptor comprising IL10RA and IL10RB, which activates multiple pathways that contribute to the downstream anti-inflammatory effects. It also plays important roles in other processes such as sterile wound healing, autoimmunity, cancer, and homeostasis. Given the wide spectrum of the anti-inflammatory properties attributed to IL-10, both IL-10 enhancing and blockade strategies have been tried in a number of diseases and cancer therapy.

Amino acid sequence: Ser 19 – Asn 178

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans