Human CellExp™ IL-13, Human Recombinant

Biomolecule/Target: IL13

Synonyms: IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13

Alternates names: IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13

Taglines: A mediator of allergic inflammation and disease

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3596

NCBI Gene Symbol: IL13

Gene Source: Human

Accession #: P35225

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: > 92%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Human IL-13, Tag Free at 10 μg/mL (100 μL/well) can bind Anti-IL13 MAb, Human IgG4 with a linear range of 0.5-8 ng/mL.

Results: N/A

Binding Capacity: Immobilized Human IL-13, Tag Free at 10 μg/mL (100 μL/well) can bind Anti-IL13 MAb, Human IgG4 with a linear range of 0.5-8 ng/mL.

Unit Definition: N/A

Molecular Weight: 13.4 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans