Human CellExp™ Insulin R / CD220, Human Recombinant

Biomolecule/Target: Insulin R / CD220

Synonyms: INSR, Insulin receptor, IR, CD220

Alternates names: INSR, Insulin receptor, IR, CD220

Taglines: Receptor tyrosine kinase which mediates the pleiotropic actions of insulin.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 3667

NCBI Gene Symbol: IRS1

Gene Source: Human

Accession #: P06213-2

Recombinant: TRUE

Source: HEK 293 cells

Purity by SDS-PAGE #: >90% as determined by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Human Insulin R (28-944), His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag with a linear range of 0.078-1.25 μg/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 106.5 kDa (His 28 - Lys 944)

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. It is recommended to store at -20°C.

Background Information: Insulin receptor (INSR) is also known as CD antigen CD220, which can be cleaved into the following 2 chains: Insulin receptor subunit alpha and Insulin receptor subunit beta. INSR is a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains carry the insulin-binding regions, while the beta chains carry the kinase domain. Forms a hybrid receptor with IGF1R, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short of INSR has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, INSR binds IGF1.

Amino acid sequence: AA His 28 - Lys 944

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans