Human CellExp™ LEFTY-I, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Recombinant LEFTY-I

Alternates names: Left-right determination factor 1, Left-right determination factor B, Protein lefty-1, Protein lefty-B

Taglines: Plays a role in left-right asymmetry determination of organ systems during development

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 10637

NCBI Gene Symbol: LEFTY1

Gene Source: Human

Accession #: O75610

Recombinant: Yes

Source: Human 293 cells

Purity by SDS-PAGE #: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/μg

Activity (Specifications/test method): N/A

Biological activity: The specific activity was determined by dose dependent ability to inhibit BMP-4 (6.5 ng/mL) induction of alkaline phosphatase production in the MC-3T3-E1 cell line (Mouse Chondrogenic cell line).

Results: Typically 10 to 40 ng/mL ED50.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized in 10 mM Tris-HCl, pH 7.4, 50 mM NaCl, 0.5% CHAPS.

Reconstitution Instructions: Reconstitute in sterile water.

Background Information: BioVision’s LEFTY-1 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 38 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media. Lefty proteins function as an antagonist of the Nodal Signaling pathway. Proper functioning of Lefty is crucial to the proper development of the heart, lungs, spleen, and liver.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans