Human CellExp™ TNF-alpha, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Human Cellexp Human Recombinant TNF-alpha

Alternates names: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Taglines: A potent lymphoid factor that exerts cytotoxic effects on a wide range of cells

Taglines: USA

Country of Animal Origin: China

NCBI Gene ID #.: 7124

NCBI Gene Symbol: TNF

Gene Source: Human

Accession #: P01375

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 μg of protein

Activity (Specifications/test method): N/A

Biological activity: ED₅₀ is typically < 0.9 ng/mL. The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.

Results: < 0.9 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17 kDa, non-disulfide bonded homotrimer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized in PBS.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Background Information: Tumor Necrosis Factor-α (TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. TNF is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans