Human CellExp™CNTFR/CNTFR-alpha, human recombinant

Biomolecule/Target: CNTFR/CNTFR-alpha

Synonyms: CNTFR, CNTF R alpha

Alternates names: CNTFR, CNTF R alpha

Taglines: This receptor and its cognate ligand support the survival of neurons.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 1271

NCBI Gene Symbol: CNTFR

Gene Source: Human

Accession #: P26992

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per μg of the rh CNTFR alpha by the LAL method.

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human CTNFR at 10 μg/ml (100 μl/well) can bind biotinylated human CNTF with a linear ranger of 3.2 - 100 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein rh CNTFR alpha is fused with polyhistidine tag at the C-terminus, has a calculated MW of 36.9 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 45-48 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Background Information: Ciliary neurotrophic factor receptor subunit alpha (CNTFR or CNTFR-alpha) belongs to the type I cytokine receptor family and type 3 subfamily. CNTFR contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. This receptor and its cognate ligand support the survival of neurons. This receptor is most closely related to the interleukin-6 receptor. This receptor possesses an unusual attachment to the cell membrane through a glycophosphatidylinositol linkage.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans