Human CellExp™ TNFRSF10B /TRAILR2, human recombinant

Biomolecule/Target: TNFRSF10B /TRAILR2

Synonyms: TNFRSF10B, TRAILR2, TRAIL-R2, CD262, DR5, KILLER, TRICK2, ZTNFR9, TRICKB

Alternates names: TNFRSF10B, TRAILR2, TRAIL-R2, CD262, DR5, KILLER, TRICK2, ZTNFR9, TRICKB

Taglines: A receptor for the cytotoxic ligand TNFSF10/TRAIL

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 8795

NCBI Gene Symbol: TNFRSF10B

Gene Source: Human

Accession #: O14763

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 μg/ml (100 μl/well) can bind biotinylated TNFSF10 with a linear range of 0.375 - 10 ng/ml.

Results: Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF10B at 10 μg/ml (100 μl/well) can bind biotinylated TNFSF10 with a linear range of 0.375 - 10 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein fused with 6×His tag at the C-terminus, has a calculated MW of 15.1 kDa. The predicted N-terminus is Ile 56. DTT-reduced Protein migrates as 15-20 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Tumor necrosis factor receptor superfamily member 10B (TNFRSF10B) also known as TNF-related apoptosis-inducing ligand receptor 2 (TRAILR2), Death receptor 5 (DR5), CD262, KILLER, is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TNFRSF10B / DR-5 is widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines. TRAILR2 / CD262 / DR5 is the receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD (a death domain containing adaptor protein) of TRAIL-R2 / TNFRSF10B recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. CD262 / DR5 Promotes the activation of NF-kappa-B. DR5 is essential for ER stress-induced apoptosis and is regulated by p53/TP53.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans