Human Cellxp™ Her3 / ErbB3, Fc Tag, Human Recombinant

Biomolecule/Target: N/A

Synonyms: ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

Alternates names: ERBB3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3

Taglines: Involved in pathways leading to cell proliferation or differentiation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2065

NCBI Gene Symbol: ErbB3

Gene Source: Human

Accession #: P21860

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >90%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Human NRG1-beta1 Protein (ECD, Fc Tag) at 2 μg/mL (100 μL/well) can bind Human ErbB3, Mouse IgG2a Fc Tag with a linear range of 0.3-2 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 95.5 kDa. The protein migrates as 115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: ErbB3,also known as Her3 (human epidermal growth factor receptor 3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain. It therefore can bind the ligand but cannot mediate the intracellular signal transduction through protein phosphorylation. However, it does form heterodimers with ErbB2 or other EGFR members responsible for tyrosine phosphorylation to give a receptor complex and initiate the related pathway, which lead to cell proliferation or differentiation. Overexpression of this protein has been reported in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

Amino acid sequence: Ser 20 - Thr 643

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans