Human CellExp™ B7-1 / CD80, mouse recombinant

Biomolecule/Target: CD80

Synonyms: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

Alternates names: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7

Taglines: Plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 12519

NCBI Gene Symbol: Cd80

Gene Source: Mouse

Accession #: Q00609

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Immobilized Mouse B7-1, His Tag at 2μg/Ml (100 μL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.

Results: N/A

Binding Capacity: Immobilized Mouse B7-1, His Tag at 2μg/Ml (100 μL/well) can bind Mouse CTLA-4, Fc Tag with a linear range of 0.12-0.5 ng/mL.

Unit Definition: N/A

Molecular Weight: 25.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to a concentration of 50 μg/ml.

Background Information: B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA- 4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans