Human CellExp™ DNAM-1 / CD226, Fc Tag, Human Recombinant

Biomolecule/Target: N/A

Synonyms: DNAM1, CD226, PTA1

Alternates names: DNAM1, CD226, PTA1

Taglines: Mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 10666

NCBI Gene Symbol: CD226

Gene Source: Human

Accession #: NP_006557

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: >95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Rhesus macaque CD155 at 5 μg/mL (100 μL/well) can bind Human DNAM-1, Fc Tag with a linear range of 2-31 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.6 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: DNAX accessory molecule 1 (DNAM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DNAM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Amino acid sequence: AA Glu 19 - Asn 247

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans