Human CellExp™ E-Cadherin /ECAD /Cadherin-1/CD324 Protein, human recombinant

Biomolecule/Target: Cadherin-1

Synonyms: Cadherin-1, CDH1, CD324, E-cadherin, Arc-1, ECAD, LCAM, UVO

Alternates names: Cadherin-1, CDH1, CD324, E-cadherin, Arc-1, ECAD, LCAM, UVO

Taglines: Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 999

NCBI Gene Symbol: CDH1

Gene Source: Human

Accession #: P12830

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/μg by LAL method

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 67 kDa. The predicted N-terminus is Gln 23. DTT-reduced Protein migrates as 67 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Background Information: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans