Human CellExp™ Erythropoietin Receptor

Biomolecule/Target: N/A

Synonyms: EPOR, EPO-R

Alternates names: EPOR, EPO-R

Taglines: Mediates erythropoietin-induced erythroblast proliferation and differentiation

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2057

NCBI Gene Symbol: N/A

Gene Source: Human

Accession #: P19235, NP_000112.1

Recombinant: 1

Source: HEK293 cells

Purity by SDS-PAGE #: ≥ 95% by SDS-PAGE

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 EU/μg of the protein as determined by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 60-300 ng/ml in the presence of 0.2 U/ml of Recombinant Human EPO Receptor/EPOR.

Results: Measured by its ability to inhibit Epo-dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 60-300 ng/ml in the presence of 0.2 U/ml of Recombinant Human EPO Receptor/EPOR.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The protein migrates as a band at ~55-60 kDa band in SDS-PAGE.

Concentration: N/A

Appearance: Lyophilized

Physical form description: N/A

Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/ml using sterile distilled water

Background Information: Erythropoietin Receptor is a member of the cytokine receptor family. It mediates erythropoietin-induced erythroblast proliferation and differentiation, activation of Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor plays a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis.

Amino acid sequence: The target protein is expressed with the sequence (Ala25-Pro250) of human Erythropoietin Receptor protein fused to Fc and 6×His tag at the C-terminus.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans