Human CellExp™ ICOSLG /B7-H2 /CD275, Fc Tag, Human Recombinant

Biomolecule/Target: N/A

Synonyms: ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand

Alternates names: ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand

Taglines: Ligand for the T-cell-specific cell surface receptor ICOS

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 23308

NCBI Gene Symbol: ICOSLG

Gene Source: Human

Accession #: O75144

Recombinant: FALSE

Source: HEK 293 cells

Purity by SDS-PAGE #: >95% as determined by SDS-PAGE.

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Activity (Specifications/test method): Immobilized Human ICOS (C136S, C137S) at 5 μg/mL (100 μL/well) can bind Human B7-H2, Fc Tag with a linear range of 0.3-5 ng/mL

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 53.1 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. It is recommended to store at -20°C.

Background Information: ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

Amino acid sequence: AA Asp 19 - Ser 258

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans