Human CellExp™ LRRC32 / GARP, Fc Tag, Human Recombinant

Biomolecule/Target: LRRC32

Synonyms: GARP, Garpin, D11S833E, Glycoprotein A repetitions predominant

Alternates names: GARP, Garpin, D11S833E, Glycoprotein A repetitions predominant

Taglines: Binds to TGF-beta to suppress naïve T cell proliferation.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 2615

NCBI Gene Symbol: LRRC32

Gene Source: Human

Accession #: Q14392

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGE #: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/μg

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Immobilized Latent TGFB1, N-His at 5 μg/mL (100 μL/well) can bind Human LRRC32, Fc Tag with a linear range of 0.039-2.5 μg/mL.

Results: N/A

Binding Capacity: Immobilized Latent TGFB1, N-His at 5 μg/mL (100 μL/well) can bind Human LRRC32, Fc Tag with a linear range of 0.039-2.5 μg/mL.

Unit Definition: N/A

Molecular Weight: 92.6 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Background Information: Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans