Human CellExp™ Renin, Human Recombinant

Biomolecule/Target: N/A

Synonyms: Angiotensinogenase, REN.

Alternates names: Angiotensinogenase, REN.

Taglines: An aspartyl protease specific for angiotensinogen.

Taglines: USA

Country of Animal Origin: USA

NCBI Gene ID #.: 5972

NCBI Gene Symbol: REN

Gene Source: Human

Accession #: P00797

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE #: ≥95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): The specific activity is > 20 U/mg. The specific activity of active Renin was assayed using BioVision’s Renin Activity Fluorometric Assay Kit (Catalog # K800).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of active human Renin is defined as the amount of enzyme that generates 1.0 µmol of substrate per min under the assay conditions at 37 ºC.

Molecular Weight: 43 kDa (C terminal 8×His tag)

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: N/A

Background Information: Renin is a highly specific endopeptidase with the known function of generating angiotensin I from angiotensinogen in the plasma. This enzymatic reaction with the particular substrate angiotensinogen initiates a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits much lower level of enzymatic activity relative to activated renin. BioVision’s active human renin is produced from the pro-form prorenin by removing the 43 amino acid N-terminal pro-segment with trypsin

Amino acid sequence: Leu 67 – Arg 406

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans